(2000) Far-eastern blotting seems to have been first named in 2000 by Ishikawa & Taki.in 1994, which they originally dubbed TLC blotting, and was based on a similar method introduced by Towbin in 1984. This method is based on earlier work by Taki et al. The method involved blotting of phospholipids on polyvinylidene fluorideor nitrocellulose membrane prior to transfer of proteins onto the same nitrocellulose membrane by conventional western blotting and probing with conformation specific antibodies. (1996) Eastern-western blot was first used by Bogdanov et al.The immobilized RNA is then probed using DNA. (1984) Middle-eastern blotting has been described as a blot of polyA RNA (resolved by agarose) which is then immobilized.(1982) The term eastern blotting was specifically rejected by two separate groups: Reinhart and Malamud referred to a protein blot of a native gel as a native blot Peferoen et al., opted to refer to their method of drawing sodium dodecyl sulfate-gel separated proteins onto nitrocellulose using a vacuum as Vacuum blotting.In some cases, the technique had been in practice for some time before the introduction of the term. The current definitions are summarized below in order of the first use of the name however, all are based on some earlier works. All of the definitions are a derivative of the technique of western blot developed by Towbin in 1979. History and multiple definitions ĭefinition of the term eastern blot is somewhat confused due to multiple sets of authors dubbing a new method as eastern blot, or a derivative thereof. In principle, eastern blotting is similar to lectin blotting (i.e., detection of carbohydrate epitopes on proteins or lipids). Eastern blotting should be used to refer to methods that detect their targets through specific interaction of the post-translational modifications and the probe, distinguishing them from a standard far-western blot. Transferred proteins are analyzed for post-translational modifications using probes that may detect lipids, carbohydrate, phosphorylation or any other protein modification. Multiple techniques have been described by the term "eastern blot(ting)", most use phosphoprotein blotted from sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gel on to a polyvinylidene fluoride or nitrocellulose membrane. Thus, eastern blot can be considered an extension of the biochemical technique of western blot. It is most often used to detect carbohydrate epitopes. The eastern blot, or eastern blotting, is a biochemical technique used to analyze protein post-translational modifications including the addition of lipids, phosphates, and glycoconjugates.
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